CHM 1310 - General Chemistry I
CHM 1315 - General Chemistry I Laboratory
CHM 3300 - Survey of Biochemistry
CHM 3450 - Biochemistry I
CHM 3455 - Biochemistry Laboratory
CHM 3460 - Biochemistry II
CHM 4860 - Biochemsitry III/Advanced Biochemistry
CHM 5180 - Bioanalytical Problem Solving
CHM 5700 - Energy Chemsitry
B.Sc.(Hon), University of Peradeniya (Sri Lanka), 1996
Ph.D., Miami University (Ohio), 2004
Postdoctoral Fellow, Dept of Biophysics, Medical College of Wisconsin, Milwaukee
American Society for Biochemistry and Molecular Biology (ASBMB)
My research focuses on all aspects of hydrolytic enzymes, especially Zn-metalloproteases and glycoside hydrolases in order to understand their physiological function, in disease development and molecular evolution of bacterial life in oligotrophic environments.
My research interest spans across several disciplines of biological chemistry, focusing on the following areas:
Bacterial oligotrophic metabolism
Within my research, I use molecular biological/cloning techniques in bacterial and yeast expression systems to obtain the proteins of interest. Easy-to-handle eukaryotic model systems, such as C. elegans, functional genomic techniques, are employed to understand the functional significance of these metalloenzymes in eukaryotes.
Periyannan GR. Bacterial Cellobiose Metabolism: An Inquiry-Driven, Comprehensive Undergraduate Laboratory Teaching Approach to Promote Investigative Learning. Biochem Mol Biol Educ. 2019 Mar 28.
Gamage DG, Gunaratne A, Periyannan GR, Russell TG. Applicability of Instability Index for In vitro Protein Stability Prediction. Protein Pept Lett. 2019 Feb 28.
Gopal R. Periyannan, Barbara A. Lawrence, Annie E. Egan. 1H-NMR Based Conformational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity. J. Chem. Educ. 2015, 92 (7), pp 1244–1249
Presley, G. N., Payea M. J., Hurst L. R., Egan A. E., Martin, B. S., & Periyannan, G. R. (2014). Extracellular gluco-oligosaccharide degradation by Caulobacter crescentus. Microbiology, 160 (Pt3):635-45.
Tioni MF, Llarrull LI, Poeylaut-Palena AA, Martí MA, Saggu M, Periyannan GR, Mata EG, Bennett B, Murgida DH, Vila AJ, " Trapping and Characterization of a Reaction Intermediate in Carbapenem Hydrolysis by B. cereus Metallo-beta-lactamase" J Am Chem Soc. (2008)130 (47), 15852–15863.
Hu, Z., Periyannan, G., Bennett, B., and Crowder, M.W., “Role of the Zn1 and Zn2 sites in metallo-β-lactamase L1” J Am Chem Soc. (2008) Oct 29;130(43):14207-16.
Z. Hu, G.R. Periyannan, M.W. Crowder, "Folding strategy to prepare Co(II)-substituted metallo-beta-lactamase L1" Anal Biochem. (2008)Jul 15;378(2):177-83.
Amit Kumar, Gopal Raj Periyannan, Beena Narayanan, Aaron W. Kittell, Jung-Ja Kim and Brian Bennett. Experimental evidence for a metallohydrolase mechanism in which the nucleophile is not delivered by a metal ion: EPR spectrokinetic and structural studies of aminopeptidase from Vibrio proteolyticus. Biochem J.(2007) May 1;403(3):527-36.
M.L. Matthews, G. Periyannan, T.K. Sigdel, C. Hajdin, B. Bennett, and M.W. Crowder, “Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid- freeze quench EPR studies on the Co(II)-substituted enzyme” J. Am. Chem. Soc.(2006) Oct 11;128(40):13050-1.
A. L. Costello, G. R. Periyannan, K.-W. Yang, M. W. Crowder, D. L. Tierney, "Site selective binding of Zn(II) to metallo-ß-lactamase L1 from Stenotrophomonas maltophilia," JBIC 2006, 11(3), 351-8.
G. R. Periyannan, A. L. Costello, D. L. Tierney, K.-W. Yang, B. Bennett, M.W. Crowder, "Sequential binding of Co(II) to metallo-ß-lactamase CcrA," Biochemistry 2006, 45(4), 1313-1320.
G. P. K. Marasinghe, I. M. Sander, B. Bennett, G. R Periyannan, K.-W. Yang, C. A. Makaroff, M. W. Crowder, "Structural studies on a mitochondrial glyoxalase II," J. Biol. Chem. 2005, 280(49), 40668-40675.